The Agilent Feature Extraction software with default settings (user manual version ) was used to obtain normalized expression values from the raw scans. The software package GeneSpring GX v (Agilent Technologies) was used for quality control checks, data normalization by the 75th percentile method. See the Feature Extraction User Guide to learn the purpose of all the parameters and settings and how to modify them. When a protocol is assigned to an extraction set, the software loads a set of protocol parameter values and settings that affect the process and results for Feature Extraction. Agilent protocols are meant for use. The data summarization procedures used in the Agilent Feature Extraction software allow for the summarization of the multiple probes and probe replicates into a robust measurement for each microRNA. This measurement, the "TotalGeneSignal,” is found in both of the Feature Extraction output files: the full text and “GeneView” files.
Agilent's feature extraction software automatically reads and processes up to raw microarray image files. The software finds and places microarray grids, rejects outlier pixels, accurately determines feature intensities and ratios, flags outlier pixels, and calculates statistical confidences. View and Download Agilent Feature Extraction Software Version (FE ) Release Notes instruction manual online. Agilent Feature Extraction software extracts data from microarray images produced in the following different situations: † Agilent microarrays scanned on an Agilent scanner † Non-Agilent microarrays scanned on an Agilent scanner † Agilent microarrays scanned on a supported non-Agilent scanner (i.e.
Agilent's Feature Extraction software automatically reads and processes up to raw microarray image files. The software finds and places microarray grids. sophisticated feature extraction software named. Mapix®. buffer (Agilent) and hybridised to OGT's microarray slides. Screen protocol. Agilent Technologies. Agilent. Feature Extraction User Guide. For Research Use Only. Not for use in diagnostic procedures.
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